Severe acute respiratory syndrome-related coronavirus-2 (SARS–CoV-2) is a novel coronavirus responsible for the current pandemic of coronavirus disease-2019 (COVID-19) leading to respiratory tract infections including adult respiratory distress syndrome (ARDS). Microbiology confirmation with a real-time polymerase chain reaction (RT-PCR) is essential for diagnosis of COVID-19 disease. Real-time polymerase chain reaction has an important role to play in individual patient management as well as in part of pandemic control measures like isolation, quarantine, and containment. Serological methods for antibody detection play an important role in population surveillance and do not have much role in the diagnosis of the disease.
Li Z, Yi Y, Luo X, et al. Development and clinical application of a rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis. J Med Virol 2020. DOI: 10.1002/jmv.25727.
Joynt GM, Wu WK. Comment understanding COVID-19: what does viral RNA load really mean? Lancet Infect Dis 2020. DOI: 10.1016/S1473-3099(20)30237-1.
Tang Y-W, Schmitz JE, Persing DH, et al. The laboratory diagnosis of COVID-19 infection: current ssues and challenges. J Clin Microbiol 2020;4:JCM.00512–JCM.00520.
World Health Organization (WHO). Rapid communication on the role of the GeneXpert® platform for rapid molecular testing for SARS-CoV-2 in the WHO European Region European Laboratory Initiative on TB, HIV and Viral Hepatitis. Copenhagen;1st April 2020.
Okba NMA, Müller MA, Li W, et al. Severe acute respiratory syndrome coronavirus 2-specific antibody responses in coronavirus disease 2019 patients. Emerg Infect Dis 2020;26(7). DOI: 10.3201/eid2607.200841.
Zhao J, Yuan Q, Wang H, et al. Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019. SSRN Electron J 2020. ciaa344. DOI: 10.1093/cid/ciaa344.
Che X, Qiu L, Liao Z, et al. Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43. J Infect Dis 2005;191(12):2033–2037. DOI: 10.1086/430355.
Bruning AHL, Leeflang MMG, Vos JMBW, et al. Rapid tests for influenza, respiratory syncytial virus, and other respiratory viruses: a systematic review and meta-analysis. Clin Infect Dis 2017;65(6):1026–1032. DOI: 10.1093/cid/cix461.