The laboratory plays a crucial role in diagnosing fungal infections that are often underdiagnosed. Identifying invasive fungal infections in the immunocompromised population requires a high index of suspicion and choice of diagnostic methods is of utmost importance. This article aims to describe the various methods in a nutshell and hence explain how an integrated approach helps in accurate diagnosis which will benefit the patient. It also illustrates a few interesting case reports along with their laboratory findings.
Retrieved from https://mycology.adelaide.edu.au/mycoses/.
Seth R, Xess I, Jana M. Diagnosis of invasive fungal infections in children. Indian Pediatr 2019;56(3):229–236. DOI: 10.1007/s13312-019-1505-7.
Wattier RL, Dvorak CC, Hoffman JA, et al. A prospective, international cohort study of invasive mold infections in children. J Pediatr Infect Dis Soc 2014;4(4):313–322. DOI: 10.1093/jpids/piu074.
Kurade SM, Amladi SA, Miskeen AK. Skin scraping and potassium hydroxide mount. Indian J Dermatol Venereol Leprol 2006;72(3):238–241. DOI: 10.4103/0378-6323.25794.
Abassi M, Boulware DR, Rhein J. Cryptococcal meningitis: diagnosis and management update. Curr Trop Med Rep 2015;2(2):90–99. DOI: 10.1007/s40475-015-0046-y.
Azar MM, Hage CA. Laboratory diagnostics for histoplasmosis. J Clin Microbiol 2017;55(6):1612–1620. DOI: 10.1128/JCM.02430-16.
Guarner J, Brandt ME. Histopathologic diagnosis of fungal infections in the 21st century. Clin Microbiol Rev 2011;24(2):247–280. DOI: 10.1128/CMR.00053-10.
Hageage GJ, Harrington BJ, The use of calcofluor white in clinical mycology. Abstr 269 21st Interscience Conference on Antimicrobial Agents and Chemotherapy, 1981.
Retrieved from https://mycology.adelaide.edu.au/descriptions/hyphomycetes/aspergillus/.
Maxfield L, Matthews JJ, Ambrosetti DR, et al. Trichosporon fungemia in a pediatric patient with acute lymphoblastic leukemia. ID Cases 2015;2(4):106–108. DOI: 10.1016/j.idcr.2015.09.007.
Al-Sweih N, Ahmad S, Joseph L, et al. Malassezia pachydermatis fungemia in a preterm neonate resistant to fluconazole and flucytosine. Med Mycol Case Rep 2014;5:9–11. DOI: 10.1016/j.mmcr.2014.04.004.
Ghosh I, Raina V, Kumar L, et al. Serum galactomannan assay for diagnosis of probable invasive aspergillosis in acute leukemia and hematopoietic stem cell transplantation. Indian J Med Paediatr Oncol 2013;34(2):74–79. DOI: 10.4103/0971-5851.116181.
Lamoth F. Galactomannan and 1,3-β-d-glucan testing for the diagnosis of invasive aspergillosis. J Fungi (Basel). 2016;2(3):22. DOI: 10.3390/jof2030022.
Al-Juaid A, Al-Rezqi A, Almansouri W, et al. Pediatric gastrointestinal basidiobolomycosis: case report and review of literature. Saudi J Med Med Sci 2017;5(2):167–171.
Wang Y, Li D, Qiao L, et al. Infant central nervous system aspergillosis with first-episode of intracranial hemorrhage: a case report. Medicine (Baltimore) 2017;96(47):e8893. DOI: 10.1097/MD.0000000000008893.
