Molecular techniques constitute valuable tools to improve the diagnosis of bacterial infections in children when cultures remain negative, etiologies of which would otherwise go nondocumented. The clinical utility of such tests could be optimized by systematic prescription by the pediatricians after consulting the microbiologist.
Lim PPC, Stempak LM, Malay S, et al. Determining the clinical utility of 16S rRNA sequencing in the management of culture-negative pediatric infections. Antibiotics (Basel) 2022;11(2):159. DOI: 10.3390/antibiotics11020159
Patel A, Harris KA, Fitzgerald F. What is broad-range 16S rDNA PCR? Arch Dis Child Educ Pract Ed 2017;102(5):261–264. DOI: 10.1136/archdischild-2016-312049
Filleron A, Michon AL, Jumas-Bilak E, et al. What to expect from molecular tools for non-documented pediatric infectious diseases. Expert Rev Mol Diagn 2015;15(12):1645–1656. DOI: 10.1586/14737159.2015.1105132
Lin KP, Yeh TK, Chuang YC, et al. Blood culture negative endocarditis: a review of laboratory diagnostic approaches. Int J Gen Med 2023;16:317–327. DOI: 10.2147/IJGM.S393329
Naureckas Li C, Nakamura MM. Utility of broad-range PCR sequencing for infectious diseases clinical decision making: a pediatric center experience. J Clin Microbiol 2022;60(5):e0243721. DOI: 10.1128/jcm.02437-21
Le Monnier A, Carbonnelle E, Zahar JR, et al. Microbiological diagnosis of empyema in children: comparative evaluations by culture, polymerase chain reaction, and pneumococcal antigen detection in pleural fluids. Clin Infect Dis 2006;42(8):1135–1140. DOI: 10.1086/502680
