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VOLUME 2 , ISSUE 2 ( April-June, 2020 ) > List of Articles

Notes From The Lab

Laboratory Diagnosis of Severe Acute Respiratory Syndrome-related Coronavirus-2 Infection

Aruna Poojary

Citation Information : Poojary A. Laboratory Diagnosis of Severe Acute Respiratory Syndrome-related Coronavirus-2 Infection. Pediatr Inf Dis 2020; 2 (2):70-74.

DOI: 10.5005/jp-journals-10081-1234

License: CC BY-NC 4.0

Published Online: 01-09-2019

Copyright Statement:  Copyright © 2020; The Author(s).


Severe acute respiratory syndrome-related coronavirus-2 (SARS–CoV-2) is a novel coronavirus responsible for the current pandemic of coronavirus disease-2019 (COVID-19) leading to respiratory tract infections including adult respiratory distress syndrome (ARDS). Microbiology confirmation with a real-time polymerase chain reaction (RT-PCR) is essential for diagnosis of COVID-19 disease. Real-time polymerase chain reaction has an important role to play in individual patient management as well as in part of pandemic control measures like isolation, quarantine, and containment. Serological methods for antibody detection play an important role in population surveillance and do not have much role in the diagnosis of the disease.

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  1. WHO. Novel coronavirus—China. Accessed April 23, 2020.
  2. Zhu N, Zhang D, Wang W, et al. A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med 2020;382(8):727–733. DOI: 10.1056/NEJMoa2001017.
  3. Li Q, Guan X, Wu P, et al. Early transmission dynamics in Wuhan, China, of novel coronavirus-infected pneumonia. N Engl J Med 2020;382(13):1199–1207. DOI: 10.1056/NEJMoa2001316.
  4. Siordia JA. Epidemiology and clinical features of COVID-19: a review of current literature. J Clin Virol 2020;127:104357. DOI: 10.1016/j.jcv.2020.104357.
  8. He X, Lau EH, Wu P, et al. Temporal dynamics in viral shedding and transmissibility of COVID-19. Nat Med 2020;26(5):672–675. DOI: 10.1038/s41591-020-0869-5.
  9. Wölfel R, Corman VM, Guggemos W, et al. Virological assessment of hospitalized patients with COVID-2019. Nature 2020. DOI: 10.1038/s41586-020-2196-x.
  10. World Health Organization (WHO). Laboratory testing for coronavirus disease 2019 (COVID-19) in suspected human cases. Interim Guid. 2020;(March):1–7.
  12. World Health Organization (WHO). Infection prevention and control of epidemic- and pandemic-prone acute respiratory infections in health care. WHO Guidel. 2014;1–156.
  13. European Center for Disease Control (ECDC). Infection prevention and control for COVID-19 in healthcare settings–first update 12 March 2020.
  14. Corman VM, Landt O, Kaiser M, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill 2020;25(3):2000045. DOI: 10.2807/1560-7917.ES.2020.25.3. 2000045.
  15. Corman V, Bleicker T, Brünink S, et al. Diagnostic Detection of Wuhan Coronavirus 2019 by Real-Time RT-PCR. Geneva: World Health Organization; 2020.
  16. Cheng MP, Papenburg J, Desjardins M, et al. Diagnostic testing for severe acute respiratory syndrome-related coronavirus-2: a narrative review. Ann Intern Med 2020. M20–1301. DOI: 10.7326/M20-1301.
  17. Wong ML, Medrano JF. Real-time PCR for mRNA quantitation. BioTechniques 2005;39(1):75–85. DOI: 10.2144/05391RV01.
  18. Pfaffl MW. Quantification strategies in real-time PCR. In: Bustin SA. A-Z of quantitative PCR. La Jolla, San Diago CA, USA: International University Line (IUL; 2004. pp. 87–112.
  19. Jiang G, Ren X, Liu Y, et al. Application and optimization of RT-PCR in diagnosis of SARS-CoV-2 infection. medRxiv 2020.
  21. Li Z, Yi Y, Luo X, et al. Development and clinical application of a rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis. J Med Virol 2020. DOI: 10.1002/jmv.25727.
  22. Joynt GM, Wu WK. Comment understanding COVID-19: what does viral RNA load really mean? Lancet Infect Dis 2020. DOI: 10.1016/S1473-3099(20)30237-1.
  23. Tang Y-W, Schmitz JE, Persing DH, et al. The laboratory diagnosis of COVID-19 infection: current ssues and challenges. J Clin Microbiol 2020;4:JCM.00512–JCM.00520.
  24. World Health Organization (WHO). Rapid communication on the role of the GeneXpert® platform for rapid molecular testing for SARS-CoV-2 in the WHO European Region European Laboratory Initiative on TB, HIV and Viral Hepatitis. Copenhagen;1st April 2020.
  25. Okba NMA, Müller MA, Li W, et al. Severe acute respiratory syndrome coronavirus 2-specific antibody responses in coronavirus disease 2019 patients. Emerg Infect Dis 2020;26(7). DOI: 10.3201/eid2607.200841.
  26. Zhao J, Yuan Q, Wang H, et al. Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019. SSRN Electron J 2020. ciaa344. DOI: 10.1093/cid/ciaa344.
  27. Che X, Qiu L, Liao Z, et al. Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43. J Infect Dis 2005;191(12):2033–2037. DOI: 10.1086/430355.
  30. Bruning AHL, Leeflang MMG, Vos JMBW, et al. Rapid tests for influenza, respiratory syncytial virus, and other respiratory viruses: a systematic review and meta-analysis. Clin Infect Dis 2017;65(6):1026–1032. DOI: 10.1093/cid/cix461.
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